HSF1 interacted directly with both of the N-terminal sequences of the Ku70 and Ku86 proteins, which inhibited the endogenous heterodimeric interaction between Ku70 and Ku86. The blocking of the Ku70 and Ku86 interaction by HSF1 induced defective NHEJ repair activity and ultimately activated genomic instability after ionizing radiation (IR), which was similar to effects seen in Ku70 or Ku80

Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80. The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase.

The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). Two of these proteins, Ku70 and Ku80, were identified by immunoblot analysis. The third polypeptide, which was identified by mass spectrometry analysis, is identical to poly(ADP-ribose) polymerase-1(PARP-1), a 113-kDa enzyme that functions as a sensor of DNA damage. 55K isoform of CDK9 associates with Ku70 and is involved in DNA repair. Liu H., Herrmann C.H., Chiang K., Sung T.L., Moon S.H., Donehower L.A., Rice A.P. Positive elongation factor b (P-TEFb) is a cellular protein kinase that is required for RNA polymerase II (RNAP II) … Invitrogen Anti-Ku70 Recombinant Monoclonal (JM61-31), Catalog # MA5-32645.

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The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Mutant mice deficient in Ku70 exhibit early aging. Using several specific criteria of aging, the mutant mice were found to display the same aging signs as control mice, but at a considerably earlier chronological age. Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.

Invitrogen Anti-Phospho-Ku70 (Ser5) Polyclonal, Catalog # PA5-40247. Tested in Western Blot (WB) applications. This antibody reacts with Human, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL).

Involved in DNA non- homologous end joining (NHEJ) required for double-strand break repair. Oct 6, 2020 4941, Sequence mapping, 1, The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70  The C-terminal region consists of a 5 kDa SAP domain (Ku70-SAP) which involved in DNA binding during NHEJ reaction. Expression, Ku70 is ubiquitously   Homo sapiens KU70(XRCC6) and KU80(XRCC5) protein sequences were retrieved from. UniProt and KU 70/80 beta barrel domain sequence length was  Rabbit anti Ku70 antibody recognizes human Ku70, also known as XRCC6, G22P1 or X-ray repair cross-complementing protein 6.

(DNA-PKcs) that is recruited to DNA ends by the Ku70/Ku80 heterodimer. Q54T85); DNA-dependent protein kinase catalytic subunit(UniProt - Q54UC0) 

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Ku70 binding activity was localized to the C-terminal coiled-coil domain of nCLU. Leucine residues 357, 358, and 361 of nCLU were necessary for Ku70-nCLU interaction. The N- and C-terminal coiled-coil domains of nCLU interacted with each other, suggesting that the protein could dimerize or fold. IHC-plus™ XRCC6 / Ku70 Antibody LS-B5387 is a pathologist validated IHC antibody Mouse anti-Human XRCC6 / Ku70. Validated for ELISA,IF,IHC,WB.
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Liu H., Herrmann C.H., Chiang K., Sung T.L., Moon S.H., Donehower L.A., Rice A.P. Positive elongation factor b (P-TEFb) is a cellular protein kinase that is required for RNA polymerase II (RNAP II) … Invitrogen Anti-Ku70 Recombinant Monoclonal (JM61-31), Catalog # MA5-32645. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human samples.

Liu H., Herrmann C.H., Chiang K., Sung T.L., Moon S.H., Donehower L.A., Rice A.P. Positive elongation factor b (P-TEFb) is a cellular protein kinase that is required for RNA polymerase II (RNAP II) transcriptional elongation of protein coding genes. Hypertonic treatment inhibits radiation-induced nuclear translocation of the Ku proteins G22p1 (Ku70) and Xrcc5 (Ku80) in rat fibroblasts.
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Ku70 has been referred to by several names including: Lupus Ku autoantigen protein p70; ATP-dependent DNA helicase 2 subunit 1; X-ray repair complementing 

Mon. MW, 69.8 kDa. UniProt, P12956  X-ray repair complementing defective repair in Chinese hamster cells 6 . Gene name : XRCC6;.

Rabbit anti Ku70 antibody recognizes human Ku70, also known as XRCC6, G22P1 or X-ray repair cross-complementing protein 6. Ku70 is involved in repair of 

Chai W., Ford L.P., Lenertz L., Wright W.E., Shay J.W. Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). Two of these proteins, Ku70 and Ku80, were identified by immunoblot analysis.

Key Links. Genomic Location Protein Family (UniProt). Belongs to the ku70 family. (Q23976). Protein Signatures (InterPro). Description. X-ray repair cross complementing 6 [Source:HGNC Symbol;Acc: HGNC:4055].